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目的:观察Par-4基因在新生大鼠脑缺氧缺血后,不同脑组织中的表达。方法:制备新生大鼠缺氧缺血性脑病的动物模型。利用RT-PCR检测脑缺氧缺血后不同时间点海马组织、大脑额叶皮质及小脑中Par-4的mRNA表达的改变,利用Westernblot检测在缺氧缺血24h后,海马组织大脑额叶皮质和小脑中Par-4蛋白表达量的改变。结果:①海马组织和大脑额叶皮质中的Par-4mRNA在脑缺氧缺血后2h已明显升高,至6h已达高峰。而小脑未见表达;②在新生大鼠脑缺氧缺血24h后,海马组织及大脑额叶皮质中Par-4蛋白表达量显著升高,并且海马组织中Par-4蛋白表达量高于大脑额叶皮质中Par-4蛋白表达量。而小脑未见其蛋白表达。结论:缺氧缺血对新生大鼠不同脑组织中Par-4基因表达影响不同,Par-4可能参予了缺氧缺血对新生大鼠海马组织和大脑额叶皮质的致病过程。
Objective: To observe the expression of Par-4 gene in different brain tissues after hypoxic-ischemic brain damage in neonatal rats. Methods: An animal model of neonatal hypoxic-ischemic encephalopathy was established. The mRNA expression of Par-4 in the hippocampus, the frontal cortex and the cerebellum were detected by RT-PCR at different time points after hypoxia and ischemia. Western blot was used to detect the expression of Par-4 in the hippocampus And Par-4 protein expression in cerebellum. Results: (1) The Par-4 mRNA in hippocampus and the frontal cortex of the hippocampus significantly increased at 2h after hypoxic-ischemic brain injury and peaked at 6h. But no expression in the cerebellum. (2) The expression of Par-4 protein in hippocampus and the frontal cortex of hippocampus was significantly increased 24 h after hypoxic-ischemic brain damage in neonatal rats, and the expression of Par-4 protein in hippocampus was higher than that in brain Par-4 protein expression in frontal cortex. The cerebellum did not see its protein expression. CONCLUSION: Hypoxia-ischemia has a different effect on Par-4 gene expression in different brain tissues of neonatal rats. Par-4 may be involved in the pathogenic process of hypoxic-ischemic brain damage in the hippocampus and the frontal cortex of neonatal rats.