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用紫外分光光度法和荧光光谱法研究了多柔比星(Adriamycin,ADM)稀土金属离子配合物(ADM-M)与DNA的相互作用。结果发现,在pH=7.0时,ADM与Eu3+、Yb3+能形成稳定配合物,该配合物可使DNA的最大吸收产生明显的减色效应及红移,并能够竞争置换溴化乙锭(EB)与DNA的结合点。KI猝灭试验发现DNA对ADM-M有明显的保护作用,热变性后的DNA对ADM-M的荧光猝灭程度减弱,离子强度的改变使ADM-M-DNA体系的荧光强度增加。实验结果表明:ADM-M与DNA之间主要为嵌入和静电作用方式。
The interaction between Adriamycin (ADM) rare earth metal ion complex (ADM-M) and DNA was studied by UV spectrophotometry and fluorescence spectroscopy. The results showed that ADM and Eu3 + and Yb3 + could form a stable complex at pH = 7.0. The complex could remarkably reduce the maximum absorption of DNA and redshift, and could competitively displace ethidium bromide (EB) The point of binding to DNA. KI quenching assay showed that DNA had a significant protective effect on ADM-M. The fluorescence quenching of ADM-M by heat-denatured DNA was weakened, and the change of ionic strength increased the fluorescence intensity of ADM-M-DNA system. The experimental results show that the main intercalating and electrostatic interaction modes between ADM-M and DNA.