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为研究细胞角蛋白(CK)在病毒性肝炎肝纤维化发生中的意义,我们对49例病毒性肝炎病人肝组织进行了病理及 CK 免疫组织化学研究,同时对其肝组织进行了α-平滑肌肌动蛋白(α-SMA)、层粘蛋白(LN)、Ⅳ型胶原(Ⅳ-C)以及血清Ⅳ-C、LN 检测,结果报道如下。一、病人及诊断标准49例病人为我院住院病毒性肝炎患者,其中乙型肝炎37例,甲型肝炎3例,丙型肝炎6例,重叠感染3例。肝脏穿刺留取肝组织标本,10%甲醛固定,石蜡包埋。切片厚0.5μm,分别做 HE染色及免疫组化。临床及病理诊断标准,按照1995年北京第五次全国传染病与寄生虫病学术会议修订的诊断标准。二、主要试剂及方法免疫组化主要试剂及方法:抗人 CK、α-SMA、Ⅳ-C、LN等抗体,北京中山生物技术有限公司分装美国 ZYMED 公司产
In order to study the significance of cytokeratin (CK) in the pathogenesis of hepatic fibrosis in viral hepatitis, we studied the pathology and CK immunohistochemistry of liver tissues in 49 patients with viral hepatitis, (Α-SMA), laminin (LN), collagen Ⅳ (Ⅳ-C) and serum Ⅳ-C, LN were detected. The results are reported as follows. First, the patient and diagnostic criteria 49 patients were hospitalized patients with viral hepatitis, including 37 cases of hepatitis B, hepatitis A in 3 cases, hepatitis C in 6 cases, 3 cases of overlapping infections. Liver biopsy specimens of liver tissue, 10% formaldehyde fixed, embedded in paraffin. Slice thickness 0.5μm, respectively, HE staining and immunohistochemistry. Clinical and pathological diagnostic criteria, in accordance with the 1995 Beijing Fifth National Conference on Infectious Diseases and Parasitic Diseases revised diagnostic criteria. Second, the main reagents and methods Immunohistochemical reagents and methods: anti-human CK, α-SMA, Ⅳ-C, LN and other antibodies, Beijing Zhongshan Biotechnology Co., Ltd. American ZYMED production company