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目的:建立稳定可靠的兔胰头癌动物模型,选择最佳的模型制作方法。方法:48只兔采用随机区组方法分为两组,每组24只,分别采用瘤块悬液法、瘤块植入法将肿瘤细胞注射至胰头内制作兔胰腺癌动物模型,计量数据比较采用双因素完全随机设计方差分析,分别于1、2、3和4周处死大白兔行移植组织解剖和病理学检查,观察2种不同建模方式的肿瘤生长情况及生物学形态。结果:建模过程中,2种方法成瘤率均为100%。瘤块植入法术后1周肿瘤体积为(0.90±0.13)cm3,离散系数为0.14;第2周肿瘤体积为(3.44±0.41)cm3,离散系数为0.12;第3周肿瘤体积为(7.53±0.69)cm3,离散系数为0.09;第4周肿瘤体积为(36.75±5.93)cm3,离散系数为0.16。瘤块悬液法术后1周肿瘤体积为(1.15±0.21)cm3,离散系数为0.18;第2周肿瘤体积为(4.94±1.64)cm3,离散系数为0.33;第3周肿瘤体积为(9.47±4.26)cm3,离散系数为0.45;第4周肿瘤体积为(46.31±16.77)cm3,离散系数为0.36。对术后肿瘤体积进行比较:1)瘤块植入法离散系数较小,生长相对稳定;2)生长时间对肿瘤生长体积具有显著影响,F=78.569 4,P=0.002 4;而生长时间与建模方法的交互作用对肿瘤生长体积无显著影响,P>0.05。术后第1周两组动物均未发现转移,瘤块植入法第2周未见种植性转移,第3周5只大白兔(83.33%)大网膜出现转移,瘤块悬液法第2周1只大白兔(16.67%)出现结肠、肓肠及腹壁种植转移,第3周4只大白兔(66.67%)出现结肠、盲肠及腹壁种植转移,2只大白兔(33.33%)出现大网膜转移。微观病理显示,瘤块与种植前瘤块病理形态基本一致,大部分瘤块悬液法HE染色下图像可见中间有较多坏死组织。瘤块植入法较瘤块悬液法肿瘤生长更为稳定。结论:成功建立了兔胰头癌动物模型,瘤块植入法是建立兔胰头癌动物模型的较佳方法。
Objective: To establish a stable and reliable animal model of pancreatic head pancreatic cancer and choose the best model making method. Methods: Forty-eight rabbits were randomly divided into two groups (24 rats in each group). The tumor cells were injected into the pancreas of the pancreas to make animal model of pancreatic adenocarcinoma. The measurement data Comparison of two-factor randomized design analysis of variance, respectively, at 1, 2, 3 and 4 weeks after the death of the white rabbits transplantation anatomical and pathological examination to observe the two different modes of tumor growth and biological morphology. Results: In the modeling process, the tumor formation rates of the two methods were all 100%. The tumor volume was (0.90 ± 0.13) cm3 and the coefficient of dispersion was 0.14 at 1 week after tumor implantation. The tumor volume at the second week was (3.44 ± 0.41) cm3 and the discrete coefficient was 0.12. The tumor volume at the third week was (7.53 ± 0.69) cm3, the coefficient of dispersion was 0.09; the volume of tumor in the fourth week was (36.75 ± 5.93) cm3, and the coefficient of dispersion was 0.16. The tumor volume was (1.15 ± 0.21) cm3 at 1 week after the operation, and the discrete coefficient was 0.18. The tumor volume at the second week was (4.94 ± 1.64) cm3 and the discrete coefficient was 0.33. The tumor volume at the third week was (9.47 ± 4.26) cm3, the coefficient of dispersion was 0.45; the volume of tumor in the fourth week was (46.31 ± 16.77) cm3, the coefficient of dispersion was 0.36. The tumor volume was compared after operation: 1) The tumor growth rate was relatively stable with small discrete coefficient of growth and tumor growth; 2) growth time had a significant effect on tumor growth volume (F = 78.569 4, P = 0.002 4) The interaction of modeling methods had no significant effect on tumor growth volume, P> 0.05. No metastasis was observed in the two groups at the first week after operation. No implant metastasis was observed in the second week of tumor implantation. In the third week, the omentum of 5 rabbits (83.33%) showed metastasis. The colon, colon and abdominal wall metastasized in 1 rabbits (16.67%) at 2 weeks. The colon, cecum and abdominal wall were transplanted in 4 rabbits (66.67%) in the third week, and large rabbits (33.33% Omental metastasis. Microscopic pathology showed that the pathological form of the tumor mass was similar to that of the tumor mass before planting. Most of the tumor mass under the HE staining showed more necrotic tissue in the middle. Tumor mass implantation method is more stable than tumor mass suspension tumor growth. Conclusion: The animal model of pancreatic head cancer in rabbit has been successfully established. The best method to establish animal models of pancreatic head cancer in rabbits is tumor mass implantation.