Sixty-five natural gas samples were collected from 19 oil-gasfields in the Qaidam basin, China. The chemical composition and carbon isotope values of the samples were measured, and the geochemical characteristics and origin of the natural gases were studied. The gases can be divided into biogenic gases, sapropelic oil-type gases, mixed type oil-type gases, coal-type gases and mixed gas. The δ13C1 values of the biogenic gases are very small and the C2+ contents of them are very low, ranging from -68.2‰ to -61.8‰ and 0.06% to 0.20% respectively. They have heavy δD and δ13CCO2, showing a CO2 reduction pathway. They are distributed in the East depression region and derived from the Quaternary source rocks. The sapropelic oil-type gases have small δ13C2 values and high C2+ ranging from -36.6‰ to -28.6‰ and from 33.01% to 47.15% respectively. The mixed type oil-type gases have δ13C2 values and C2+ contents varying from -28.6‰ to -24.8‰ and from 4.81% to 26.06% respectively. Both sapropelic oil-type gases and mixed type oil-type gases are associated with oil-type oils, distributed in the West depression region and derived from the Tertiary saltwater lacustrine sapropelic source rocks and humic source rocks respectively. The δ13C2 values of the coal-type gases are extremely high and the C2+ contents are very low, changing from -23.3‰ to -12.5‰ and from 0.06% to 18.07% respectively. The coal-type gases in the Nanbaxian gasfield and the Lenghu oil-gasfields in the North fault block belt are derived from the Middle Jurassic coal-measures source rocks, whereas those in the West depression region are derived from the Tertiary saltwater lacustrine humic source rocks. Compared with some other basins in China, the natural gases there have obviously heavier δ13C due to the heavier δ13C of different types of kerogens of the Tertiary saltwater lacustrine source rocks in the West depression region of the basin. The mixing of natural gases is common in the West depression region, but the mixed gases are formed by sapropelic oil-type gases, mixed type oil-type gases or coal-type gases, respectively, of different levels of maturity. Most of the sapropelic oil-type gases and mixed type oil-type gases in the west part are thermally immature and low-mature, but the coal-type gases in the West depression region and the North fault block belt are mature and high- to over-mature.