论文部分内容阅读
质型多角体病毒(cypoviruses,CPV)是昆虫常见病原微生物。采用生物信息学方法对7种昆虫CPV基因组编码序列进行密码对使用模式分析,探究昆虫CPV基因组特征。分析结果表明:昆虫CPV基因组中密码对的使用是非随机的,密码对组合NNA→ANN、NNG→GNN、NNU→CNN、NNU→UNN中的多数密码对是CPV负偏好性密码对,密码对组合NNA→CNN、NNA→UNN、NNG→CNN、NNU→GNN中的多数密码对是CPV正偏好性密码对;残值最高的10个密码对与残值最低的10个密码对中有4个共用密码子(AAA、AAG、GAU、UAU),进一步说明密码对使用的偏好性;不同CPV间的基因组密码对使用偏好性程度相近,但极端偏好性密码对种类不同。同一电泳型CPV在聚类分析中的距离较近,说明同一电泳型CPV的基因组密码对使用模式相似,并且系统发育分析结果与基于密码对使用模式的聚类分析结果一致,说明CPV基因组密码对使用模式分析可用于研究其基因组进化。昆虫CPV基因组密码对使用具有偏好性,这种偏好性是其基因组特征的一种表现。
Cytoplasmic polypodies (CPV) are common pathogenic microorganisms in insects. Bioinformatics methods were used to analyze the CPV genome coding sequences of seven species of insects to explore the characteristics of the insect CPV genome. The results showed that the use of the pair of ciphers in the insect CPV genome was nonrandom. The pair of ciphertexts in the combination of NNA → ANN, NNG → GNN, NNU → CNN, and NNU → UNN were negative CPP pairs, Most pairs of pairs in NNA → CNN, NNA → UNN, NNG → CNN, NNU → GNN are CPV positive preference pairs; 10 pairs with the highest salvage value share the same 4 out of 10 pairs with the lowest salvage value The codons (AAA, AAG, GAU, UAU) further illustrate the preference of the password for use; the genomic code between different CPVs is similar to the preference, but the extreme preference password pairs are different in kind. The same electrophoretic CPV in the cluster analysis of the distance closer, indicating that the same electrophoresis CPV genome code on the use of similar patterns, and phylogenetic analysis results based on the use of patterns and password-based clustering analysis shows that the CPV genome code pairs Use pattern analysis can be used to study its genomic evolution. Insect CPV genome codes have a preference for use, which is a manifestation of their genomic characteristics.