论文部分内容阅读
目的 研究喉癌浸润的淋巴细胞的分离及培养技术,为开展喉癌的过继免疫治疗提供手段。方法 将15份喉鳞癌标本用胰蛋白酶消化,进行淋巴细胞分离,用含白介素-2的培养液培养,扩增,装安瓿,冷冻保存备用。在此期间对细胞悬液中细胞成分的变化作了对比分析,并分析白介素-2在淋巴细胞分离中的作用。结果 从喉的不同部位摘取的肿瘤组织及肿瘤不同分期的淋巴细胞培养后分离出的数量无差异。冷冻保存对淋巴细胞活力无影响。白介索-2是淋巴细胞的生长的重要条件。结论 正确按照此法培养的喉癌浸润的淋巴细胞能达到临床应用水平。
Objective To study the technique of separating and culturing laryngeal cancer infiltrating lymphocytes and to provide a means for adoptive immunotherapy of laryngeal cancer. Methods Fifteen samples of laryngeal squamous cell carcinoma were digested with trypsin and lysed. Lymphocytes were isolated and cultured in culture medium containing interleukin-2. Ampoules were ampoules and cryopreserved for later use. During this period, the change of cell components in the cell suspension was compared and analyzed, and the role of interleukin-2 in lymphocyte separation was analyzed. Results There was no difference in the numbers of isolated tumor tissues from different parts of the larynx and lymphocytes cultured in different stages of the tumor. Cryopreservation has no effect on lymphocyte viability. White mediated cable -2 is an important condition for the growth of lymphocytes. Conclusion The laryngeal cancer infiltrating lymphocytes cultured correctly according to this method can reach the clinical application level.