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目的:观察不同强度游泳训练后大鼠机体贮存铁及十二指肠铁吸收相关蛋白的变化,探讨不同强度运动对机体内铁贮存及铁吸收的影响机制。方法:60只雌性SD大鼠随机分为对照组(controlgroup,CG),适度运动组(moderatelyexercisedgroup,MG)和过度运动组(strenuouslyexercisedgroup,SG),每组20只,分别于运动5周和10周后取材。采用酶联免疫吸附法(ELISA)检测血清铁蛋白(serumferritin,SF)含量;测定肝脏和骨髓非血红素铁含量;采用WesternBlot法检测十二指肠上皮细胞二价金属离子转运体1(divalentmetaltransporter1,DMT1)和膜铁转运蛋白1(ferroportin1,FP1)的变化。结果:(1)运动10周后,MG组大鼠SF和Hb显著高于SG组和CG组(P<0.05),而SG组大鼠SF显著低于CG组(P<0.05);MG组和SG组大鼠骨髓非血红素铁含量均显著低于CG组(P<0.01)。(2)MG组十二指肠上皮细胞DMT1(IRE)和FP1表达均显著高于CG组(P<0.05),而SG组DMT1和FP1表达与CG组和MG组相比均无显著差异。结论:适度运动可增加十二指肠铁转运蛋白表达,促进肠铁吸收,使血清铁增多、贮存铁重新分布,满足运动中机体对铁的需求;而过度运动使贮存铁大量消耗,从而易引发非贫血性铁缺乏。
OBJECTIVE: To observe the changes of iron-related iron absorption and iron-related absorption of duodenum in rats after swimming with different intensities of swimming, and to explore the mechanism of different intensity exercise on iron storage and iron absorption in the body. Methods: Sixty female Sprague-Dawley rats were randomly divided into control group (CG), moderately exercised group (MG) and strenuouslyexercisedgroup (SG), with 20 rats in each group. After drawing. The levels of serum ferritin (SF) were measured by enzyme-linked immunosorbent assay (ELISA). The contents of non-heme iron in liver and bone marrow were measured. The divalent metal ion transporter 1 DMT1) and ferroportin1 (FP1). Results: (1) After 10 weeks of exercise, SF and Hb in MG group were significantly higher than those in CG group and CG group (P <0.05), while SF group in SG group was significantly lower than CG group (P <0.05) And non-heme iron in bone marrow of SG group were significantly lower than CG group (P <0.01). (2) The expressions of DMT1 (IRE) and FP1 in MG group were significantly higher than those in CG group (P <0.05), while the expression of DMT1 and FP1 in SG group were not significantly different from CG group and MG group. Conclusions: Moderate exercise can increase duodenal iron transporter protein expression, promote intestinal iron absorption, increase serum iron and redistribute stored iron to meet the body’s demand for iron during exercise. Excessive exercise consumes large amount of stored iron, making it easy Lead to non-anemic iron deficiency.