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目的:从自由基代谢方面观察大蒜素对高同型半胱氨酸血症的影响。方法:Wistar雄性大鼠分为大蒜素高剂量组、大蒜素低剂量组、叶酸对照组、模型对照组和空白对照组,饲养6周,腹腔静脉取血,测定血清同型半胱氨酸和SOD、MDA,数据进行统计学分析。结果:模型对照组在6周时血浆同型半胱氨酸水平高于空白对照组和大蒜素高、低剂量组;模型对照组和叶酸对照组的血清超氧化物岐化酶(SOD)水平低于空白对照组(P<0.05),大蒜素高剂量组和大蒜素低剂量组的血清SOD水平明显高于模型对照组(P<0.05),叶酸对照组的血清SOD水平与模型对照组差异无显著性(P>0.05);模型对照组和叶酸对照组的血清丙二醛(MDA)水平显著高于空白对照组(P<0.05),大蒜素高剂量组和大蒜素低剂量组的血清MDA水平明显低于模型对照组(P<0.05),大蒜素高剂量组和大蒜素低剂量组的血清MDA水平也明显低于叶酸对照组(P<0.05),叶酸对照组的血清MDA水平与模型对照组比较差异无显著性(P>0.05)。结论:大蒜素能明显降低高同型半胱氨酸血症大鼠血中的同型半胱氨酸水平,并升高高同型半胱氨酸血症大鼠血清SOD活性,降低高同型半胱氨酸血症大鼠血清MDA水平,提示大蒜素能明显对抗高同型半胱氨酸血症引起的脂质过氧化反应,从而对血管内皮细胞发挥保护作用。
OBJECTIVE: To observe the effect of allicin on hyperhomocysteinemia from the aspects of free radical metabolism. Methods: Wistar male rats were divided into high-dose allicin group, low-dose allicin group, folic acid control group, model control group and blank control group. The rats were bred for 6 weeks. Blood samples were obtained from the abdominal vena cava. Serum homocysteine and SOD , MDA, data for statistical analysis. Results: The levels of plasma homocysteine in model control group were higher than those in blank control group and allicin high and low dose groups at 6 weeks. The levels of serum superoxide dismutase (SOD) in model control group and folic acid control group were low In the blank control group (P <0.05), the levels of serum SOD in the allicin high-dose group and the low-dose allicin group were significantly higher than those in the model control group (P <0.05). The levels of serum SOD in the folic acid control group were not significantly different from those in the model control group (P <0.05). The level of serum malondialdehyde (MDA) in the model control group and the folic acid control group was significantly higher than that in the blank control group (P <0.05). The levels of serum MDA (P <0.05). The levels of serum MDA in high-dose garlicin group and low-dose allicin group were significantly lower than those in the folic acid control group (P <0.05). The levels of MDA in the folic acid control group were significantly lower than those in the model control group There was no significant difference in the control group (P> 0.05). CONCLUSION: Allicin can significantly reduce homocysteine levels in hyperhomocysteinemia rats and increase serum SOD activity in hyperhomocysteinemia rats, and decrease high homocysteine The level of MDA in the serum of the rats with acidosis suggests that allicin can obviously antagonize the lipid peroxidation reaction induced by hyperhomocysteine and thus exert a protective effect on the vascular endothelial cells.