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目的：探讨细胞因子（ＴＮＦα及ＩＬ－６）水平与ＨＢＶ前Ｃ区变异之间的关系。方法：应用ＥＬＩＳＡ法及３′－碱基特异性聚合酶链反应法对４３例ＨＢＶＤＮＡ阳性慢性乙型肝炎患者进行血清ＴＮＦα、ＩＬ－６含量及ＨＢＶ前Ｃ区１８９６位点突变的检测。结果：ＨＢＶ前Ｃ区突变株，检出率为４８．８％（２１／４３），突变株感染组ＴＮＦα、ＩＬ－６水平明显高于野生株感染组。结论：慢性乙型肝炎患者血清ＴＮＦα及ＩＬ－６水平与ＨＢＶ前Ｃ区基因变异有密切关系，机体免疫压力的增高可能是导致ＨＢＶ前Ｃ区变异的重要原因之一
Objective: To investigate the relationship between the levels of cytokines (TNFα and IL-6) and the variation of pre-HBV C region. Methods: Serum levels of TNFα and IL-6 were measured in 43 patients with HBVDNA-positive chronic hepatitis B by ELISA and 3’-base-specific polymerase chain reaction (PCR). Results: The positive rate of pre-HBV mutants was 48.8% (21/43). The levels of TNFα and IL-6 in the mutants were significantly higher than those in the wild-type infected ones. Conclusion: The serum levels of TNFα and IL-6 in patients with chronic hepatitis B are closely related to the gene mutation in pre-C region of HBV, and the increase of immune pressure may be one of the important causes of pre-HBV mutation