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采用ＤｙｎａｌＭ－４５０ＣＤ＿（３４）免疫磁珠分离纯化脐血ＣＤ＿（３４）￣＋细胞，并探讨了不同细胞因子［干细胞因子（ＳＣＦ）、白细胞介素６（ＩＬ－６）、白细胞介素３（ＩＬ－３）及红细胞生成素（Ｅｐｏ）］组合对脐血ＣＤ＿（３４）￣＋细胞的体外培养和扩增作用。结果表明：①经ＤｙｎａｌＭ－４５０ＣＤ＿（３４）免疫磁珠分离的脐血ＣＤ＿（３４）￣＋细胞，其纯度达８５％～。９５％；②甲基纤维素培养体系中，在不同组合的细胞因子作用下，脐血ＣＤ＿（３４）￣＋细胞的集落产率明显不同，单用ＩＬ－６最低，ＳＣＦ、ＩＬ－６、ＩＬ－３及Ｅｐｏ联用产率最高；③在低氧液体培养体系中，ＩＬ－６和ＩＬ６＋Ｅｐｏ不能有效地扩增脐血ＣＤ＿（３４）￣＋细胞，而其它细胞因子组合均可产生明显的扩增作用；其中加Ｅｐｏ的组合扩增效果显著高于无Ｅｐｏ的相应组合。脐血ＣＤ＿（３４）￣＋细胞分离、纯化及其体外扩增的研究对于脐血造血细胞库的建立，脐血造血细胞移植和基因导入的研究均具有重要意义。
The CD34 + cells of umbilical cord blood were isolated and purified by using DynalM-450CD_ (34) immunomagnetic beads. The effects of different cytokines (SCF, IL-6, IL- IL-3) and erythropoietin (Epo)] on cord blood CD34 (34) cells in vitro culture and expansion. The results showed that: ① The purity of CD_ (34) ~ + cord blood cells isolated by DynalM-450CD_ (34) immunomagnetic beads was 85% ~. 95%, respectively. (2) In the methylcellulose culture system, the yield of CD34 (34) cells in umbilical cord blood was significantly different under the different combination of cytokines. The single use of IL-6 alone, SCF, IL- IL-3 and Epo combined with the highest yield; ③ In hypoxic liquid culture system, IL-6 and IL6 + Epo can not effectively expand cord blood CD_ (34) ~ + cells, while other cytokines combination can produce significant Amplification effect; which combination of Epo amplification effect was significantly higher than the corresponding combination without Epo. Isolation, Purification and Expansion of CD34 (+) Cells in Umbilical Cord Blood It is of great significance to establish a cord blood hematopoietic cell bank, to study cord blood hematopoietic cells transplantation and gene introduction.