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[目的]研究丛枝菌根对黄檗氮素及含氮物质含量的影响。[方法]通过盆栽试验,用丛枝菌根真菌接种黄檗一年生实生苗,研究丛枝菌根对黄檗(Phellodendron amurense Rupr).氮素及含氮物质含量的影响。[结果]黄檗幼苗形成丛枝菌根后,增加了叶片氮素含量,接种G.mosseae的苗木叶片氮素含量比对照提高了1.28~1.60倍,光合能力增强。接种丛枝菌根菌后,显著提高了黄檗叶绿素含量及叶绿素a和b的比值,增强了黄檗光合作用的能力,叶绿素a含量提高了25%以上;同时,增加了植物体内吲哚乙酸的含量,增加比例在1.65~2.41倍之间;增强植物体内硝酸还原酶的活性,增加了可溶性蛋白含量,增加比例在1.67~2.49倍之间,增强了植物氮素代谢能力,促进植物生长,增强植物的次生代谢能力。[结论]该研究结果为丛枝菌根真菌在黄檗上的应用提供了理论依据。
[Objective] The research aimed to study the effects of arbuscular mycorrhizal fungi on the contents of nitrogen and nitrogenous substances in Huangma. [Method] The pot experiment was conducted to inoculate the annual seedlings of Curcuma longissima with arbuscular mycorrhizal fungi to study the effects of arbuscular mycorrhizal colonization on the contents of nitrogen and nitrogenous substances in Phellodendron amurense Rupr. [Result] After the arbuscular mycorrhizal fungi were formed, the nitrogen content of leaves increased after A. arborescens seedlings were inoculated with G. mosseae, and the photosynthetic capacity was increased by 1.28-1.60 times. Arbuscular mycorrhizal fungi inoculated with Arbuscular Mycorrhizal fungi significantly increased the chlorophyll content, the ratio of chlorophyll a and b, and enhanced the photosynthetic capacity of P. przewalskii. The content of chlorophyll a increased by 25%. At the same time, the content of indoleacetic acid , Increasing the proportion of 1.65 ~ 2.41 times; enhance plant nitrate reductase activity, increased soluble protein content, increasing the ratio of 1.67 to 2.49 times, enhance plant nitrogen metabolism, promote plant growth and enhance plant Secondary metabolism. [Conclusion] The results of this study provided a theoretical basis for the application of arbuscular mycorrhizal fungi on the yellow berry.