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采用 TSH单克隆抗体包被聚苯乙烯试管 ,并对抗体包被条件进行了研究 ,建立了 TSH免疫放射分析法。结果表明 :在 2～ 8℃下过夜包被 ,抗体浓度为 2 .1mg/L时 ,制备的包被管用于 TSH免疫放射分析 ,结合达到最大 ;不同浓度下包被 ,包被温度和时间对制备的包被管的影响不同。TSH免疫放射分析最小检测限为 0 .0 5 m IU/L;回收率为 88.4 %～ 10 0 .0 % ;批内、批间变异系数分别为7.4 %～ 10 .9%和 8.7%～ 10 .7%。 4 6例健康人血样测定值为 0 .3～ 6 .2 m IU/L。
TSH monoclonal antibody coated polystyrene tubes, and antibody coating conditions were studied to establish TSH immunoassay. The results showed that the coated tubes were used for TSH immunoradiometric assay with the maximum antibody concentration of 2.1 mg / L at 2 ~ 8 ℃ for overnight coating. The coating, coating temperature and time at different concentrations The effect of the prepared tube is different. The minimum detectable limit of TSH was 0. 05 mU / L, the recovery was 88.4% -10.0%, and the coefficient of variation (CV) was 7.4% -10.9% and 8.7% -10 .7%. Blood samples from 46 healthy subjects were measured at 0.3-6.2 μmol / L.