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目的1.观察原发性血小板增多症(primary thrombocytosis,PT)患者血小板功能指标CD 62P、PAC-1、TXB 2、6-keto-PGF1α及TXB 2/6-keto-PGF1α比值的变化。2.观察PT患者血小板功能指标与血小板数量的相关性。3.探讨可能导致PT患者血栓发生的机制。方法1.流式细胞仪(FCM)测定血小板表面的CD 62P,PAC-1水平。2.ELISA方法测定血浆血栓素A2(TXA2)代谢产物TXB2和前列环素(PGI2)代谢产物6-keto-PGF1α水平。3.观察和比较各组血小板功能的变化及其与血小板数量变化的关系。结果1.PT患者血小板功能指标均高于正常组,P<0.01。2.PT患者血小板功能指标变化与血小板数量相关性分析结果提示:PT患者各项血小板功能指标与血小板数目之间不存在相关性,P>0.05。结论PT患者除血小板数量显著增多外,其血小板各项功能指标亦有明显异常。研究结果表明:PT患者发生血栓的危险因素除与其血小板数目异常增多有关外,还与血小板功能异常活化(血小板活化指标CD 62P、PAC-1异常增高)及血浆TXB 2、6-keto-PGF1α平衡失调(TXB 2/6-keto-PGF1α比值异常增高)有关。而PT患者血小板功能指标与其血小板数量之间不存在相关性,故治疗原发性血小板增多症除按常规治疗予以降低血小板数量外,还应同时注意对血小板功能的干预治疗。
Objective To observe the changes of platelet function indexes CD62P, PAC-1, TXB2, 2,6-keto-PGF1α and TXB2 / 6-keto-PGF1α in patients with primary thrombocytosis (PT) To observe the correlation between platelet function index and platelet count in PT patients. 3. To investigate the possible mechanism of thrombosis in PT patients. Methods 1. Flow cytometry (FCM) was used to measure the platelet surface CD62P, PAC-1 levels. The ELISA method was used to determine the level of 6-keto-PGF1α, a metabolite of thromboxane A2 (TXA2) and prostacyclin (PGI2). To observe and compare the changes of platelet function and the relationship between platelet count and platelet count in each group. PTT patients with platelet function indicators were higher than the normal group, P <0.01.2.PT patients with changes in platelet function index and platelet count results suggest that: PT patients with various platelet function indicators and the number of platelets does not exist between Sex, P> 0.05. Conclusion In addition to a significant increase in the number of platelets in patients with PT, the various indexes of platelets also have significant abnormalities. The results showed that the risk factors of thrombosis in patients with PT were related to the abnormal increase of platelet count, abnormal platelet activation (CD62P and PAC-1 abnormalities) and plasma TXB2,6-keto-PGF1α (TXB 2/6-keto-PGF1α ratio abnormally increased). However, there is no correlation between platelet function index and platelet count in patients with PT. Therefore, in addition to reducing the number of platelets by conventional treatment, the treatment of essential thrombocythemia should also pay attention to the intervention of platelet function.