论文部分内容阅读
亲和超滤结合液质技术是一种快速、简单、有效的活性成分发现模式,尤其适用于成分组成复杂的中药提取物。通过亲和超滤可以简化活性成分的提取步骤,快速筛选具有潜在活性的小分子化合物;液质技术则可以实现活性成分的快速发现及鉴定,二者的结合在揭示中药药效物质基础以及以中药为基础的新药研发中均有重要作用。本文结合笔者近几年的研究,对亲和超滤结合液质技术在中药有效成分发现中的应用做简要综述,内容主要包括该技术的原理、特点及其应用成果与进展。
Affinity and ultrafiltration combined with liquid technology is a fast, simple and effective mode of active ingredient discovery, especially suitable for the composition of traditional Chinese medicine extract complex. By affinity ultrafiltration can simplify the extraction of active ingredients, rapid screening of potentially small molecule compounds; liquid technology can achieve the rapid detection and identification of the active ingredient, a combination of the two in the discovery of the basis of traditional Chinese medicine efficacy and to Chinese medicine-based research and development of new drugs have an important role. Based on the author’s research in recent years, this article briefly reviews the application of affinity ultrafiltration combined with liquid-mass technology in the discovery of the active ingredients of traditional Chinese medicine. The contents mainly include the principle, characteristics, achievements and progress of the technology.