论文部分内容阅读
利用农杆菌介导法将Cry1Ca#和Bar基因转化水稻恢复系R893,获得6株再生植株。经过分子鉴定,发现Bar基因和Cry1Ca#基因成功整合到水稻基因组并稳定表达。测定其中单拷贝且可育的转基因株系苗期不同器官中Cry1Ca蛋白的表达量,发现根系中最低而叶片中最高,其中最高的第6号株系叶片中含量达到35.07μg/g。获得的转基因株系对除草剂草铵膦和稻纵卷叶螟均具有良好的抗性。
The Cry1Ca # and Bar genes were transformed into rice restorer line R893 by Agrobacterium tumefaciens, and 6 regenerated plants were obtained. After molecular identification, it was found that Bar gene and Cry1Ca # gene were successfully integrated into rice genome and stably expressed. The expression of Cry1Ca protein in different organs at the seedling stage of single copy and fertile transgenic lines was determined. Among the leaves, the highest content of Cry1Ca protein in the leaves of the 6th line was 35.07μg / g. The obtained transgenic lines have good resistance to the herbicides glufosinate ammonium and rice leaf roller.