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为了研究药物对脊髓机能的影响,过去常用离子透入、局部动脉内注射、急性蛛网膜下腔注射以及脊髓动物全身给药等方法。这些方法有的既作用于脊髓,又作用于脑,不利于定位;有的不宜于长期反复使用。最近,我们参照美国威斯康星大学药学院Yaksh和Rudy(1976)的方法,建立了大鼠脊髓蛛网膜下腔慢性瘘管埋植法。此方法简便易行,通过瘘管可长期、反复给药,在动物完全清醒的状态下进行各种慢性实验观察,以进行脊髓机能的定位研究。带有瘘管的大鼠可以存活4个月以上。一、瘘管的制备植入瘘管均选用外径0.61毫米,内径0.28毫米的PE-10聚乙烯管。瘘管的长度视大鼠体重而异,我们一般选用200～300克大鼠进行实验,瘘管全长12厘米,插入7厘米,可达腰膨大上缘。为固定瘘管插入脊髓蛛网膜下腔的深度,在离瘘管下端7厘米处,套以一直径约1.5毫米,长约3.0毫
In order to study the effects of drugs on spinal cord function, iontophoresis, intra-arterial injections, acute subarachnoid injections, and systemic administration to spinal animals were used in the past. Some of these methods both act on the spinal cord, but also on the brain, is not conducive to positioning; some are not suitable for long-term repeated use. Recently, we used the method of Yaksh and Rudy (1976) of the University of Wisconsin School of Pharmacy to establish a chronic fistula implantation in the spinal cord of the rat spinal cord. This method is simple and easy, through the fistula can be long-term, repeated administration, the animals fully awake state a variety of chronic experimental observation for spinal cord localization study. Rats with fistulas can survive for more than 4 months. First, the preparation of fistula Fistula implantation were selected outer diameter 0.61 mm, 0.28 mm in diameter PE-10 polyethylene tube. Fistula length depending on the weight of rats, we generally use 200 to 300 grams of rats for experiments, fistula length of 12 cm, inserted 7 cm, up to the upper edge of lumbar enlargement. To fix the depth of fistula insertion into the spinal subarachnoid space, at a distance of 7 cm from the lower end of the fistula, a set of a diameter of about 1.5 mm, about 3.0 milliseconds