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目的:探讨人子宫颈病变组织中P53的表达及其与端粒酶hTR mRNA表达的相关关系。方法:51例宫颈病变组织中,包括宫颈炎10例,LSIL10例,HSIL20例,SCC11例,分别利用免疫组化S-P法和原位杂交法检测病变组织中P53蛋白、端粒酶hTRmRNA的表达活性。结果:P53表达在宫颈炎组与LSIL组无差别,但显著低于HSIL和SCC组;hTR基因在HSIL/SCC中的阳性表达与CC/LSIL组以及在LSIL和HSIL/SCC之间差异有统计学意义(P<0.05);P53蛋白表达与端粒酶hTRmRNA基因表达之间具有显著相关性(P=0.042,P<0.05)。结论:端粒酶hTR和P53蛋白表达及相关性对于人子宫颈癌的早期病理诊断有较好地应用价值。同时,对该肿瘤的发生、发展规律提供一定的客观依据。
Objective: To investigate the expression of P53 in human cervical lesions and its correlation with telomerase hTR mRNA expression. Methods: The expression of P53 protein and telomerase hTR mRNA in lesions of 51 cases of cervical lesions including 10 cases of cervicitis, 10 cases of LSIL, 20 cases of HSIL and 11 cases of SCC were detected by immunohistochemical SP method and in situ hybridization . Results: The expression of P53 in cervicitis group was not significantly different from that in LSIL group, but significantly lower than that in HSIL group and SCC group (P <0.05). There was statistical significance between hTR gene expression in HSIL / SCC group and CC / LSIL group and between LSIL and HSIL / SCC (P <0.05). There was a significant correlation between P53 protein expression and telomerase hTR mRNA gene expression (P = 0.042, P <0.05). Conclusion: The expression and correlation of telomerase hTR and P53 protein have a good value in early diagnosis of human cervical cancer. At the same time, the occurrence and development of the tumor provide some objective basis.