论文部分内容阅读
目的 观察成年大鼠下胸段脊髓切断术后是否出现神经纤维残留 ,以及神经纤维残留量与后肢运动功能恢复的关系。 方法 取 12只成年大鼠 ,以尖刀片沿椎管骨壁横切下胸段脊髓。术后定期观察截瘫后肢运动功能恢复情况 ,8周处死动物 ,灌注后取损伤部位及其上下节段脊髓 ,矢状冰冻切片 ,抗神经丝抗体免疫组织化学染色 ,光镜下观察有无残留神经纤维 ,计算机技术重建脊髓横断面 ,确定残留纤维的部位及其所占脊髓横断面的比例 ,并分析残留纤维数量与后肢运动功能恢复程度的相关性。 结果 42 %动物出现程度不等的后肢运动功能恢复 ,其脊髓横切处腹侧或腹外侧有数量不等的神经丝免疫反应残留纤维 ,并与后肢运动功能的恢复呈正相关关系。 结论 以尖刀片横切大鼠脊髓的常用方法 ,易造成脊髓神经纤维的残留 ,少量残留纤维即可引起后肢运动功能相当程度的恢复。因此 ,在评价脊髓损伤后的功能恢复时 ,必须首先确定损伤是否完全。
Objective To observe whether there is nerve fiber residue after thoracotomy in adult thoracic spinal cord and the relationship between nerve fiber residue and motor function recovery. Methods Twelve adult rats were used to transect the thoracic spinal cord with a sharp blade along the wall of the spinal canal. The recovery of motor function of the hindlimb hind limbs was observed regularly after operation. The animals were sacrificed at 8 weeks. The injury site and the spinal cord at the upper and lower segments were harvested after perfusion. The frozen section of sagittal and anti-neurofilament were immunohistochemically stained with or without residual nerve Fiber, computer technology to reconstruct the spinal cord cross-section to determine the residual fiber area and its proportion of spinal cord cross-section, and analysis of the number of residual fibers and hindlimb motor function recovery degree correlation. RESULTS: Forty-two percent of the animals showed hindlimb motor function recovery with varying amounts of residual neurofilament residues in the ventral or ventral part of the spinal cord transection, which was positively correlated with motor function recovery. Conclusions The common method of cutting the spinal cord with a sharp blade can easily cause the residual of spinal nerve fibers. A small amount of residual fibers can cause the recovery of motor function of the hind limbs. Therefore, in assessing functional recovery after spinal cord injury, you must first determine whether the injury is complete.