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本文报道了应用合成间日疟ＤＮＡ片段经同位素末端标记作为探针．检测了２１份经镜检确诊的间日疟病人血样，结果全部阳性．敏感性可测出０．００１３３％的原虫血症，与培养的恶性疟及正常人血没有交叉反应。随后将该探针应用到疟疾监测中，在山东采集的３１０份发热病人血和２４５份流动人口血，应用本探针检测，未出现阳性结果，经镜检复核．结果相符．在安徽省滁州市采集３５２份病灶点人群血，有２８份出现了阳性，阳性率为７．９５％。对以上血样经ＩＦＡ方法进行抗体测定，显示合成间日疟ＤＮＡ探针杂交结果与抗体阳性率具有明显的一致性。结果表明本合成间日疟ＤＮＡ探对可应用于灭疟后期的疟疾监测．特别对流动人口集中检查和传染源检索具有潜在的应用价值。
In this paper, we report the use of isotope labeled end-labeling of synthetic P. vivax DNA as a probe. Twenty-one blood samples of Vivax confirmed by microscopic examination were tested and all were positive. Susceptibility to detect parasitemia 0.00133%, and cultured falciparum and normal blood no cross-reaction. Subsequently, the probe was applied to malaria surveillance. Blood samples collected from 310 patients with fever and 245 blood samples from Shandong were detected by this probe. No positive results were observed and the results of microscopic examination were reviewed. The result is consistent. In the Chuzhou City, Anhui Province collected 352 focal spots of blood in the crowd, 28 were positive, the positive rate was 7.95%. Antibody measurement of the above blood samples by IFA method showed that there was a clear agreement between the hybridization results of the synthetic P. vivax DNA probe and the antibody positive rate. The results show that the synthesis of P. vivax malaria DNA probe can be applied to the monitoring of malaria in the late malaria phase. In particular, it has potential application value for centralized examination and source search of floating population.