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目的研究乙型肝炎病毒（ＨＢＶ）与丙型肝炎病毒（ＨＣＶ）双重感染者肝组织内两种病毒的分布特点和相互关系。方法用免疫组化和原位杂交法分别检测了ＨＢＶ的核酸及抗原和ＨＣＶ的核酸及抗原，对其中乙型肝炎核心抗原，对其中乙型肝炎核心抗原（ＨＢｃＡｇ）与ＨＣＶＲＮＡ同时阳性的组织进一步做了同一切片的双标记染色。结果ＨＢＶ和ＨＣＶ感染细胞大多各自分布于不同的肝小叶或假小叶内；即使共存于同一小叶时，多数并不感染同一细胞。结论在乙型和丙型肝炎病毒双重感染组织中，两种病毒在多数细胞内并不直接发生相互干扰；双标记法有助于分析重叠感染组织中病毒之间的相互关系。
Objective To study the distribution and correlation of the two viruses in the liver of patients with hepatitis B virus (HBV) and hepatitis C virus (HCV) infection. Methods Immunohistochemistry and in situ hybridization were used to detect the nucleic acids and antigens of HCV and the nucleic acids and antigens of HCV respectively, and further analysis was performed on the core antigens of hepatitis B and the tissues positive for hepatitis B core antigen (HBcAg) and HCV RNA Do the same piece of double-labeled staining. Results Most of HBV and HCV infected cells were distributed in different hepatic lobules or pseudolobules, respectively. Most of the infected cells did not infect the same cells even when they co-existed in the same lobules. Conclusion In the double infection of hepatitis B and C virus, the two viruses do not directly interfere with each other in most cells. The double labeling method can be used to analyze the relationship between viruses in overlapping infected tissues.