论文部分内容阅读
目的：对从西藏地区新分离的环状病毒（Ｔｉ３０１０株）进行分类和鉴定。方法：从病毒感染的ＢＨＫ１３单层细胞中提取病毒基因组ＲＮＡ，用聚丙烯酰胺凝胶电泳分析ＲＮＡ的耐ＲＮＡ酶特性和基因组ＲＮＡ特征。结果：ＲＮＡ抵抗ＲＮＡ酶消化，基因组ＲＮＡ由１０个片段组成，带形分布为３－３－２－２，相对分子质量范围在（０．７２～３．１８）×１０６，总相对分子质量为１６．８４×１０６。结论：结合该病毒的一些生物学性状，显示了西藏地区特殊生态环境下新分离病毒的独有特征，Ｔｉ３０１０可能是环状病毒属的一个新成员。
Objective: To classify and identify newly isolated circular virus (Ti3010) from Tibet. Methods: The viral genomic RNA was extracted from virus-infected BHK13 monolayer cells and analyzed for RNAi resistance and genomic RNA characteristics by polyacrylamide gel electrophoresis. Results: The RNA was digested by RNase. The genomic RNA consisted of 10 fragments with a band distribution of 3-3-2-2 and a relative molecular mass range of (0.72 ~ 3.18) × 106. The total relative molecular mass was 16.84 × 106. Conclusion: Combining with some biological characters of the virus, it shows the unique characteristics of the newly isolated virus in the special ecological environment of Tibet. Ti3010 may be a new member of the genus.