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用6个微卫星标记对中国对虾(Fenneropenaeus chinensis)的5个家系进行系谱鉴别和遗传多样性研究。6个微卫星位点中有5个位点是多态的,并在所有家系中都显示了高度的遗传差异。5个家系中,5个多态微卫星位点共发现了30个等位基因,每个位点的等位基因数在5~8之间。实验中共发现了4个家系特异性等位基因:2#家系及4#家系各1个,5#家系2个。根据已知亲本及子代基因型,可推断出5个家系中全部亲本的基因型,据此鉴别各家系。在EN0033位点,可将5#家系与其他4个家系相区别;在RS0859位点,可将3#和4#家系与其他3个家系相区分。因此,EN0033和RS0859标记可用于鉴别5#、3#和4#家系的家系特异性标记。研究表明,用5个微卫星标记,且最少用2对微卫星标记即可鉴别5个中国对虾家系。
Six microsatellite markers were used to analyze the pedigree and genetic diversity of five families of Fenneropenaeus chinensis. Five of the six microsatellite loci are polymorphic and show a high degree of genetic variation in all pedigrees. Of the 5 pedigrees, 30 alleles were found in 5 polymorphic microsatellite loci, and the number of alleles per locus was between 5 and 8. Four family-specific alleles were found in the experiment: 1 # 2 # and # 4 # families and 2 # 5 # families. Based on the known parental and offspring genotypes, the genotypes of all of the five pedigrees were deduced to identify each pedigree. At EN0033, the 5 # lineage can be distinguished from the other 4 lines; at RS0859, the 3 # and 4 # lines can be distinguished from the other three lines. Therefore, the EN0033 and RS0859 markers can be used to identify pedigree-specific markers in the 5 #, 3 # and 4 # pedigrees. Studies have shown that five Chinese shrimp pedigrees can be identified using five microsatellite markers and at least two pairs of microsatellite markers.