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目的研究IL-24对肝癌细胞在鸡胚绒毛尿囊膜(chick chorioallantoic membrane,CAM)上的血管生成和肿瘤生长的影响及其机制的初探。方法实验采用病毒感染复数(multiplicity of infection,MOI)为5的重组慢病毒IL-24和慢病毒空载体Mock分别感染肝癌细胞HepG2,24 h后接种于CAM,4 d后观察CAM上形成的肿瘤背面血管数目,并剥离肿瘤组织称其重量,继而进行Western blot检测肿瘤组织中β-catenin的表达。结果 CAM实验显示,与Mock感染的HepG2细胞相比,慢病毒IL-24感染的HepG2细胞形成的肿瘤背面血管分支数目和肿瘤重量明显下降。Western blot结果发现慢病毒IL-24感染的HepG2细胞形成的肿瘤组织中β-catenin显著低于Mock组。结论 IL-24能够抑制肝癌细胞的肿瘤血管生成和肿瘤生长,这种作用可能是通过下调β-catenin的表达来实现的。
Objective To study the effect of IL-24 on the angiogenesis and tumor growth of hepatoma cells in chick chorioallantoic membrane (CAM) and its mechanism. Methods HepG2 cells were infected with recombinant lentivirus IL-24 with a multiplicity of infection (MOI) of 5 and empty vector Mock, and then inoculated into CAMs 24 h later. The tumors formed on CAM were observed after 4 days The number of blood vessels on the back, and peeled off the tumor tissue weight, followed by Western blot detection of β-catenin in tumor tissue expression. Results CAM experiments showed that HepG2 cells infected with lentivirus IL-24 formed a significant decrease in the number of dorsal blood vessel branches and tumor weight compared with Mock-infected HepG2 cells. Western blot results showed that β-catenin in HepG2 cells infected by lentivirus IL-24 was significantly lower than that in Mock group. Conclusion IL-24 can inhibit the tumor angiogenesis and tumor growth in hepatocellular carcinoma cells, and this effect may be achieved by down-regulating the expression of β-catenin.