以CO39四叶期水稻苗的叶片为材料,提取水稻基因组DNA,通过PCR扩增获得水稻β-l,3-葡聚糖酶基因(CO39-β-l,3-Glu),经序列测定得知该基因大小为489bp,通过Blastn搜索确定该片段位于水稻10号染色体。序列比对结果表明,CO39-β-l,3-Glu与已知的水稻Gns7和大豆GlycinesojacloneGs522194aendo-β-1,3-glucanasegene,GlycinesojacloneGs464889_Bendo-β-1,3-glucanasegene三个基因的相似性分别为51.5%,47.2%和49.1%。CO39-β-l,3-Glu基因是β-l,3-葡聚糖酶基因家族的一个成员。 Rice genomic DNA was extracted from leaves of CO39 four-leaf stage rice seedlings and the rice β-1, 3-glucanase gene (CO39-β-1, 3-Glu) was obtained by PCR amplification. The size of this gene was 489bp, and it was confirmed by Blastn search that the fragment was located on chromosome 10 of rice. Sequence alignment showed that the similarity between CO39-β-1, 3-Glu and known rice Gns7 and soybean GlycinesojacloneGs522194aendo-β-1,3-glucanase gene and GlycinesojacloneGs464889_Bendo-β-1,3-glucanase gene were 51.5%, 47.2% and 49.1%. The CO39-β-1, 3-Glu gene is a member of the β-1, 3-glucanase gene family.