论文部分内容阅读
目的观察新型胆道双极射频消融导管HabibTMEndoHPB设置不同功率及持续时间的消融效果。方法在恒温下(37.0℃)使用新型胆道射频消融导管对新鲜离体猪肝进行实验,分别设置功率5、10和15W,时间60、90、120和150s。观察在不同功率/时间参数时,消融区域的温度、宏观(短轴/长轴)及微观的变化。结果设置功率5W、时间60~150s,消融区域的最高温度为42.0~54.0℃;10W、60~150s,最高温度为52.0~70.0℃;15W、60~120s,最高温度为62.0~77.0℃。10W和60、90、120及150s时,短轴长度(mm)分别为8.5±0.8、9.0±0.8、9.5±0.8及10.0±0.8;长轴长度(mm)分别为21.0±1.0、23.5±1.0、25.5±1.0及30.0±1.0。5、10、15W时,短轴和长轴长度均有小幅增大,其中功率为10W及15W时,两组差异无统计学意义(P>0.05)。镜下消融区域均出现肝组织解离、细胞间距变大、细胞广泛变性,程度随着功率和时间增加而加重,且在10W、150s及15W、120s时,出现片状液化性坏死。结论 HabibTMEndoHPB导管设置功率10W、时间90至120s进行消融比较合适,在临床实际操作中可参考本实验结果进行适当的调整。
Objective To observe the ablation effect of new biliary bipolar radiofrequency ablation catheter HabibTMEndoHPB with different power and duration. Methods The fresh porcine liver was isolated and treated with a new bile duct radiofrequency ablation catheter at constant temperature (37.0 ℃). The power was set at 5, 10 and 15 W for 60, 90, 120 and 150 s respectively. Observed at different power / time parameters, the ablation zone temperature, macroscopic (short axis / long axis) and microscopic changes. Results The maximum power was 5W for 60 ~ 150s and the maximum temperature was 42.0 ~ 54.0 ℃ in the ablation zone. The highest temperature was 52.0 ~ 70.0 ℃ in 10W and 60 ~ 150s, and the highest temperature was 62.0 ~ 77.0 ℃ in 15W and 60 ~ 120s. The minor axis lengths (mm) were 8.5 ± 0.8, 9.0 ± 0.8, 9.5 ± 0.8 and 10.0 ± 0.8 at 10W and 60, 90, 120 and 150 s, respectively; the major axis lengths were 21.0 ± 1.0 and 23.5 ± 1.0 , 25.5 ± 1.0 and 30.0 ± 1.0.5, 10 and 15W, the short axis and the long axis both increased slightly. When the power was 10W and 15W, there was no significant difference between the two groups (P> 0.05). Liver abscission occurred in the ablation area, and the intercellular distance became larger and the cells became more degenerated. The degree of aggravation was increased with the increase of power and time, and flaky liquefaction necrosis occurred at 10W, 150s and 15W, 120s. Conclusion HabibTMEndoHPB catheter set power 10W, 90 to 120s time for ablation more appropriate, in clinical practice can refer to the results of this experiment to make the appropriate adjustments.