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目的:考察银杏叶提取物(EGb761)对佐剂性关节炎(AA)大鼠的治疗作用,初步探讨银杏叶提取物治疗AA的作用机制。方法:将大鼠随机分为正常对照组,模型组,雷公藤多苷组,EGb761低、中、高(50,100,200 mg.kg-1)剂量组。弗氏完全佐剂致AA后d 12,大鼠出现继发性炎症,分别灌胃给予雷公藤多苷和EGb761,连续16 d;在不同的时间点检测大鼠继发侧关节肿胀度;致炎d 28处死大鼠,酶联免疫法检测血清中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-4(IL-4)和白细胞介素-10(IL-10)含量;光学显微镜观察关节病理变化。结果:EGb761可明显减轻继发性AA大鼠足爪的肿胀度;降低大鼠血清中IL-1β和IL-6含量;升高大鼠血清中IL-4和IL-10含量。结论:EGb761对大鼠继发性AA具有显著的治疗作用,其作用机制可能与抑制促炎因子IL-1β和IL-6的产生和释放以及促进抑炎因子IL-4和IL-10的表达相关。
Objective: To investigate the therapeutic effect of Ginkgo biloba extract (EGb761) on adjuvant arthritis (AA) in rats and to explore the mechanism of Ginkgo biloba extract in treating AA. Methods: The rats were randomly divided into normal control group, model group, tripterygium glycosides group, low, medium and high doses of EGb761 (50,100,200 mg.kg-1). Freund’s complete adjuvant caused by AA after d12, rats with secondary inflammation, respectively, intragastric administration of tripterygium glycosides and EGb761, for 16 days; at different time points detected in rats secondary joint swelling degree; induced Serum levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-4 (IL-4) and interleukin -10 (IL-10) content; optical microscopy of joint pathological changes. Results: EGb761 significantly reduced the degree of swelling in paws of secondary AA rats, decreased the levels of IL-1β and IL-6 in serum, and increased the levels of IL-4 and IL-10 in serum of rats. CONCLUSION: EGb761 has a significant therapeutic effect on secondary AA in rats. Its mechanism may be related to inhibition of the production and release of proinflammatory cytokines IL-1β and IL-6 as well as the expression of anti-inflammatory cytokines IL-4 and IL-10 Related.