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为研究中脑导水管周围灰质（PAG）与孤束核（NTS）内脏伤害性信息传递和调控之间的相互关系，采用免疫荧光组织化学方法结合荧光金（FG）逆行追踪技术，观察了大鼠NTS和PAG之间相互投射神经元在给予胃肠道伤害性刺激后的FOS表达情况。给胃肠道以1％多聚甲醛的伤害性刺激后，FOS阳性细胞主要出现于中尾段NTS的内侧亚核；在PAG内，则主要出现于足段PAG的腹外侧区。将FG微量注射于PAG后，再给予动物刺激，发现NTS内部分FG逆行标记细胞同时为FOS阳性，它们主要分布于中尾段NTS的内侧亚核，双标细胞占FG标记细胞的十分之一左右。同上，将FG注射于中尾段NTS后再施予伤害性刺激，在PAG内发现有FOS阳性的FG道标细胞，它们集中分布于尾段PAG的腹外侧区，双标细胞约占FG标记细胞的五分之一。此外，在中缝背核内也发现有一定数且的双标细胞。本文结果提示PAG可能对NTS内内脏伤害性信息的传递具有调控作用。
In order to study the relationship between visceral nociceptive transmission and regulation of midbrain periaqueductal gray (PAG) and nucleus tractus solitarius (NTS), immunofluorescence histochemistry and fluorescence gold (FG) retrograde tracing technique were used to investigate the effects of large Mutation of neurons in mouse NTS and PAG after administration of gastrointestinal noxious stimulation of FOS expression. After nociceptive stimulation with 1% paraformaldehyde in the gastrointestinal tract, FOS positive cells mainly appeared in the medial subnucleus of NTS in middle caudate and PAG in PAG. After microinjection of FG into PAG, animals were stimulated again. It was found that some FG retrogradely labeled cells in NTS were positive for FOS at the same time. They mainly distributed in the medial subnucleus of NTS in middle caudate and double-labeled cells accounted for one tenth of FG-labeled cells about. As above, FG was injected into the middle tail section of NTS and then given the nociceptive stimulus. FOS-positive FG border cells were found in the PAG. They were distributed in the ventral region of the tail PAG. The double-labeled cells accounted for about FG-labeled cells One-fifth of the In addition, in the dorsal raphe are also found in a certain number of double-labeled cells. Our results suggest that PAG may regulate the transmission of visceral nociceptive information in NTS.