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目的:研究TNF-a基因单核苷酸多态性(SNP)308G-A位点与新疆地区维吾尔族人乙型肝炎之间的关系。方法:以聚合酶链式反应--限制性内切酶长度多态性(PCR-RFLP)技术,对120例乙肝患者和120例正常对照者TNF-a基因SNP308多态性位点进行基因分型。结果:SNP308多态性位点G/G基因型和G/A基因型频率在病例组为77%和23%,正常对照组为88%和12%,2组基因型和等位基因频率分布差异有显著性(p<0.05)。结论:TNF-a基因启动子308多态性位点与新疆维吾尔族人乙肝有明显相关性。
Objective: To investigate the relationship between single nucleotide polymorphism (SNP) 308G-A of TNF-a gene and hepatitis B in Uighur Xinjiang. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to analyze gene polymorphism of SNP308 in TNF-a gene in 120 hepatitis B patients and 120 healthy controls type. Results: The frequencies of G / G and G / A genotypes in SNP308 polymorphism were 77% and 23% in the case group and 88% and 12% in the normal control group respectively. The frequencies of genotypes and alleles in the two groups The difference was significant (p <0.05). CONCLUSION: Polymorphism of TNF-a gene promoter 308 polymorphism is significantly associated with hepatitis B in Xinjiang Uighurs.