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以种植美人蕉和旱伞竹的复合垂直流人工湿地(MIL)系统的基质(分子筛、陶粒和碎石)及表流人工湿地的基质土壤为材料,采用PCR-DGGE技术研究处理农田退水的湿地中微生物群落结构的差异及其优势菌群.结果显示,MIL系统上行池中种植美人蕉、旱伞竹和不种植物对照3个处理的表层水样和上层碎石基质以及表流人工湿地3个处理的基质土样的可操作分类单元OTU(Operation Taxonomy Unit)数目都比较多(12-14),说明其微生物种类较多,微生物多样性较高,其优势种群主要为Uncultured Xylella sp.、Uncultred bacterium以及Uncultured Tolumones sp.和Acidovorax;MIL系统上行池下层的陶粒和分子筛基质的OTU数目以对照和种植旱伞竹的较高(6-11),而种植美人蕉处理最少(4-5),呈现微生物种类少、微生物多样性较低的特点,其优势种群主要有Uncultured Xylella sp.、Uncutured soil bacterium和Uncultured Sphingomon adacsas.这些结果表明垂直复合湿地系统选择种植美人蕉能提高其下层基质还原性,降低细菌多样性,有利于硝态氮的还原去除.
The matrix (molecular sieve, ceramsite and crushed stone) of composite Vertical Flow Constructed Wetland (MIL) system and the substrate soil of table flow constructed wetland were used as materials to study the treatment of farmland reclamation by PCR-DGGE The results showed that surface water samples, upper gravel matrix and surface-stream constructed wetlands of Canopy, Umbelliferae and Non-plantation were planted in the upper pool of MIL system The number of operational taxonomy units (OTUs) in treated maize soil samples was relatively high (12-14), indicating that there were more microbial species and higher microbial diversity. The dominant species were Uncultured Xylella sp. Uncultred bacterium and Uncultured Tolumones sp. And Acidovorax; the number of OTUs in ceramsite and molecular sieve matrices in the lower upslope of the MIL system was higher for controls and planted bamboos (6-11) , Showing a small number of microbial species, microbial diversity is low, the dominant species are mainly Uncultured Xylella sp., Uncutured soil bacterium and Uncultured Sphingomonas adacsas these Results show that the vertical complex wetland system to improve their growing of Canna reducing the underlying substrate, to reduce bacterial diversity, facilitate the removal of nitrate reduction.