本文报道用固绿染色鉴定细胞杀伤,然后用瑞特氏染色区别活细胞中的幼稚细胞与正常细胞;并在培养体系中加入鸭红细胞作为内标,以矫正在培养过程中细胞的崩解和增殖导致的活力误差,建立了双重染色细胞毒白血病药敏方法。本法简单,快速而廉价,需要细胞较少,且能区别被药物杀死的是否白血病细胞。本法重复性好(CV=8.6％),可望成为易为临床接受的白血病药物敏感试验方法。 In this paper, solid-green staining was used to identify cell killing. Reuter’s staining was then used to distinguish between naive cells and normal cells in live cells. Duck erythrocytes were added to the culture system as internal standards to correct cell disintegration and Proliferation-induced vitality error, established dual staining of cytotoxic leukemia drug sensitive method. This method is simple, fast and inexpensive, requires less cells, and can distinguish whether the drug is killed by leukemia cells. This method repeatability (CV = 8.6%), is expected to become susceptible to clinical acceptance of leukemia drug-sensitive test methods.