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目的探讨p38 MAPK信号通路在糖尿病肾病中的作用。方法使用不同浓度葡萄糖及p38 mAPK特异性抑制剂SB203580干预肾小球系膜细胞,采用实时荧光定量PCR法检测各组肾小球系膜细胞p38 MAPK、MMP-9和TIMP-1 mRNA的表达。结果高糖组p38 mAPK、TIMP-1 mRNA的表达明显增加,且呈浓度依赖性;而MMP-9 mRNA表达和MMP-9/TIMP-1比值与正常组相比明显下降,同时p38 MAPK抑制剂SB203580能明显逆转以上变化。结论 p38 MAPK信号通路介导了高糖诱导的肾小球系膜细胞MMP-9和TIMP-1 mRNA表达的改变,进而参与了糖尿病肾病的进展。
Objective To investigate the role of p38 MAPK signaling pathway in diabetic nephropathy. Methods Glomerular mesangial cells were treated with different concentrations of glucose and p38 mAPK specific inhibitor SB203580. The expressions of p38 MAPK, MMP-9 and TIMP-1 mRNA in mesangial cells were detected by real-time fluorescence quantitative PCR. Results The expressions of p38 MAPK and TIMP-1 mRNA in high glucose group were significantly increased and in a concentration-dependent manner, while the ratio of MMP-9 mRNA and MMP-9 / TIMP-1 was significantly decreased compared with the normal group SB203580 can significantly reverse the above changes. Conclusion The p38 MAPK signaling pathway mediates the changes of MMP-9 and TIMP-1 mRNA expression induced by high glucose in glomerular mesangial cells, which may be involved in the progression of diabetic nephropathy.