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目的:分析新疆地区哈萨克族食管癌患者人乳头瘤病毒16型(HPV16)E6基因结构的特点。方法:采用PCR技术扩增食管癌患者HPV16E6基因,其中18例哈萨克族食管癌中8例阳性,阳性率为44.4%,并对其进行了克隆及全序列分析。结果:克隆了哈萨克族食管癌HPV16E6基因,与Genebank(AF536179.1)收录的德国株相比,E6基因中氨基酸发生了变化。结论:新疆哈萨克族食管癌HPV16E6基因有一定的变异。
Objective: To analyze the characteristics of E6 gene structure of human papillomavirus type 16 (HPV16) in Kazakh esophageal cancer patients in Xinjiang region. Methods: HPV16E6 gene was amplified by polymerase chain reaction (PCR) in 8 patients with esophageal cancer, of which 8 were positive in 18 Kazakh esophageal carcinomas and the positive rate was 44.4%. The cloned and full-length sequences were analyzed. Results: The Kazakh esophageal cancer HPV16E6 gene was cloned. Compared with the German strain included in Genebank (AF536179.1), the amino acid of E6 gene was changed. Conclusion: Xinjiang Kazakh Kazakh esophageal cancer HPV16E6 gene has some variation.