论文部分内容阅读
分析不同辐射处理后“长果”猕猴桃(Actinidia longicarpa)样本的变异性,为猕猴桃诱变育种及加快猕猴桃育种进程提供理论基础。采用0(CK)、40、60、80和100 Gy~(60)Co-γ射线辐射处理“长果”猕猴桃接穗后,用ISSR分子标记技术对5种处理后的55个样品的多态性进行研究。结果表明:从100条寡聚核苷酸引物中筛选出7条引物进行标记,平均每条引物扩增出14.3条带,其中6.7条多态性带,多态性比率为46.85%;遗传相似系数为0.79~1.00,聚类分析表明,第V类为60Gy 6~8号植株,与对照的遗传距离最远,变异程度最大。因此,辐射诱变可导致猕猴桃发生显著的遗传变异,利用ISSR分析方法,可准确测定辐照样本是否为突变体。
The variability of Actinidia longicarpa samples after different radiation treatments was analyzed to provide a theoretical basis for the mutation breeding of kiwifruit and speeding up the process of kiwifruit breeding. After treatment with 0, 60, 100 and 60 Gy of Co-γ rays, the scleroderms of ’Prunus mume’ Kiwifruit were treated with ISSR molecular markers State research. The results showed that seven primers were screened out from 100 oligonucleotide primers, with an average of 14.3 bands amplified by each primer, of which 6.7 bands were polymorphic with a ratio of 46.85%. The genetic similarity The coefficient was 0.79-1.00. Cluster analysis showed that the type V of 60Gy 6-8 plants had the longest genetic distance with the control and the highest degree of variation. Therefore, radiation mutagenesis can lead to significant genetic variation in kiwifruit and the use of ISSR analysis can accurately determine whether irradiated samples are mutants.