目的建立人多肿瘤抑制基因（ｍｕｌｔｉｐｌｅｔｕｍｏｒｓｕｐ－ｐｒｅｓｓｏｒ１，ＭＴＳ１）第二外显子ｃＤＮＡ表达克隆。方法用反转录－ＰＣＲ（ＲＴ－ＰＣＲ）取得ＭＴＳ１第二外显子ｃＤＮＡ，并将其直接联接入质粒ｐＧＥＭⅳ－Ｔ中，构建为重组质粒ｐＧＥＭⅳ－ｐ１６。结果重组质粒的酶谱分析表明其酶切位点与原设计相符，插入ｃＤＮＡ－ＰＣＲ片段大小为３０７ｂｐ，与引物区间大小一致。结论所获重组质粒为ｐＧＥＭⅳ－ｐ１６设计构建 Objective To establish a cDNA clone for the second exon of multiple tumor suppressor 1 (MTS1) gene. Methods The second exon cDNA of MTS1 was obtained by reverse transcription-PCR (RT-PCR). The cDNA was directly linked to pGEMiv-T and constructed as a recombinant plasmid pGEMiv-p16. Results The zymogram analysis of the recombinant plasmid showed that the restriction site was in accordance with the original design. The size of the inserted cDNA-PCR fragment was 307 bp, which was consistent with the size of the primer region. Conclusion The recombinant plasmid obtained was designed and constructed for pGEMiv-p16