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通过水稻幼穗培养,1991-1992两年间,在5个品种(珍汕97B、红源A、包源A、W6154s,和南广占)中共获得了50株雄性不育变异株,其中R_1代有48株,R_2代有2株。在R1代,共获得5268株再生植株,雄性不育变异的平均频率为0.91%(0.83-1.08%);在R_2代(珍汕97B)发生雄性不育变异的频率为2%。本文报道了多种花粉败育类型之间可以相互转变现象,此外不育和可育之间亦可以相互转变。对离体培养产生的雄性不育变异株用一批现有CMS(Cytoplasmicmalesterile)不育系的典型保持系、恢复系进行测交,结果表明,W6154s产生的雄性育性变异株仍保持核不育的特性;红源A产生的雄性育性变异株有的可能是嵌合体,有的其败育花粉类型虽发生了变化,但其恢保关系并没有改变,有的则可能已转成类似WA型的不育材料;南广占产生的典败变异株,其恢保关系类似WA型,可能属核不育转成CMS的首例发现。
Fifty male sterile mutant strains were obtained from five varieties (Zhenshan 97B, Hongyuan A, Baoyuan A, W6154s, and Nan Guanghan) during the 1991-1992 years through rice panicle culture. Among them, R_1 There are 48 strains, R_2 generations have 2 strains. In R1 generation, 5268 regenerated plants were obtained, and the average frequency of male sterile variation was 0.91% (0.83-1.08%). The frequency of male sterile mutation in R_2 generation (Zhenshan 97B) 2%. In this paper, we report that many types of pollen abortion can change each other. In addition, infertility and fertility can also change each other. The male sterile mutant produced by in vitro culture was tested with typical maintainer lines and restorer lines of a number of existing CMS (Cytoplasmicmalesterile) male sterile lines. The results showed that male fertile mutant strains produced by W6154s still maintained nuclear infertility The male sterility mutant produced by Hongyuan A may be a chimera, and some of its abortion pollen types have changed, but the relationship between the restorers and maintainers has not changed, while others may have turned into similar WA Type of sterile material; Nan Guang accounted for the typical mutation produced by the mutant, the recovery relationship similar to the WA type, may be the first case of nuclear infertility into CMS found.