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目的:对江南卷柏不同部位提取物在体外抗柯萨奇病毒CVB3的作用进行研究,并对其抗病毒作用机制进行初步探讨。方法:采用不同部位的提取物培养CVB3感染的Hep-2细胞,观察比较细胞病变效应(CPE),72h后用MTT法测定细胞活性。结果:在Hep-2细胞体系中,江南卷柏各不同部位提取物均具有一定的抑制CVB3对细胞的致病变作用,使细胞存活率提高,其中含有脂溶性双黄酮和水溶性黄酮苷的部分是主要的活性部位。结论:江南卷柏可以作为新潜在的抗CVB3的资源植物。
OBJECTIVE: To study the anti-Coxsackie virus (CVB3) activity of extracts from different parts of southern Chinese cabbage Selaginella polymorpha and to investigate its anti-viral mechanism. Methods: Hep-2 cells infected by CVB3 were cultured with extracts of different parts. The cytopathic effect (CPE) was observed and compared. After 72 hours, the cell viability was measured by MTT assay. Results: In Hep-2 cell system, the extracts from different parts of the stem of Coprinus comatus could inhibit the pathogenicity of CVB3 to some extent and increase the cell survival rate. The extract containing fat-soluble biflavone and water-soluble flavonoid glycosides Part is the main active site. Conclusion: Selaginella polygamy can be used as a new potential resource plant for anti-CVB3.