论文部分内容阅读
运用水油提、盐析及凝胶过滤色谱法对幽门螺杆菌ＹＣ－１１株所分泌的形成空泡细胞毒素进行了提取。纯化的形成空泡细胞毒素在ＳＤＳ－聚丙烯酰胺凝胶电泳中表现为单一条带，分子量约为８７０００，在ＰＨ２．４～６．４的环境中表现高细胞毒性。
The vacuolar cytotoxin secreted by Helicobacter pylori YC-11 was extracted by water-oil extraction, salting-out and gel filtration chromatography. Purified vacuolar cytotoxins appear as a single band on SDS-polyacrylamide gel electrophoresis with a molecular weight of about 87,000 and high cytotoxicity in the environment of pH 2.4 to 6.4.