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目的分析白细胞介素-12(IL-12)、白细胞介素-18(IL-18)对单纯疱疹病毒2型(HSV-2)DNA疫苗的免疫增强效果。方法选取60只雌性BALB/c小鼠,按照随机数字表法分为观察组、对照组,各30只,分别运用IL-12、IL-18基因联合HSV-2 DNA疫苗或单独应用HSV-2 DNA疫苗免疫,末次免疫3周后,使用致死剂量攻毒试验炎症疫苗的保护作用,并检测两组小鼠脾脏T细胞增殖能力、抗HSV-2 Ig G抗体水平、调节活化正常T细胞表达与分泌的趋化因子(RANTES)、CD4+和CD8+T淋巴细胞百分比、分泌干扰素-γ(IFN-γ)和白细胞介素-4(IL-4)的T细胞百分比等指标,分析IL-12、IL-18对HSV-2 DNA疫苗的免疫增强效果。结果观察组Con A、灭活单纯疱疹病毒的SI、抗HSV-2 Ig G抗体水平、RANTES含量、抗CD4+和CD8+T淋巴细胞百分比、分泌IFN-γ的T淋巴细胞百分比均高于对照组,其分泌IL-4的T淋巴细胞百分比低于后者,差异有统计学意义(P<0.05)。HSV-2病毒攻毒实验2周后,观察组存活9只,保护率为90.00%,对照组存活4只,保护率为40.00%,两组保护率比较,差异有统计学意义(P<0.05)。结论 IL-12、IL-18基因能够明显增强HSV-2 DNA诱导小鼠产生特异性抗HSV-2的体液免疫、细胞免疫功能,是理想的免疫佐剂,值得进一步研究。
Objective To analyze the immune enhancement effects of interleukin-12 (IL-12) and interleukin-18 (IL-18) on herpes simplex virus 2 (HSV-2) DNA vaccine. Methods Sixty female BALB / c mice were randomly divided into observation group (n = 30) and control group (n = 30). The IL-12 and IL-18 DNA vaccine and HSV- DNA vaccine and immunization three weeks after the last immunization. The mice were immunized with a lethal dose of inflammatory vaccine. The proliferative ability of spleen T cells, anti-HSV-2 Ig G antibody levels and the expression of activated normal T cells (RANTES), the percentage of CD4 + and CD8 + T lymphocytes, the percentage of T cells secreting interferon-γ (IFN-γ) and interleukin-4 (IL-4) , IL-18 on HSV-2 DNA vaccine immune enhancement effect. Results The percentages of Con A, SI inactivated herpes simplex virus, anti-HSV-2 Ig G antibody, RANTES, anti-CD4 + and CD8 + T lymphocytes and IFN- γ secreting T lymphocytes in observation group were higher than those in control group , The percentage of T lymphocytes secreting IL-4 was lower than the latter (P <0.05). After two weeks of HSV-2 virus challenge, the survival rate of the observation group was 9, the protection rate was 90.00%, and the control group was 4, the protection rate was 40.00%. There was significant difference between the two groups (P <0.05) ). Conclusion The IL-12 and IL-18 genes can significantly enhance the humoral and cellular immune responses induced by HSV-2 DNA in mice induced by HSV-2 DNA, which is an ideal immunoadjuvant and deserves further study.