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目的观察爱大霉素(EM)和庆大霉素(GM)对胞浆Ca2+影响的初步机制。方法以Fura2/AM为探针测定EM和GM在不同浓度时对LLCPK1肾上皮细胞浆Ca2+的影响,同位素示踪法测定EM和GM对线粒体Ca2+摄取和内质网Ca2+摄取的影响。结果EM和GM在1mmol·L-1时对胞浆Ca2+浓度无显著影响,P>0.05;在10mmol·L-1时可使胞浆Ca2+显著上升,P<0.01。EM和GM在1mmol·L-1时显著促进线粒体Ca2+摄取(P<0.05);在10mmol·L-1时,二者显著抑制线粒体Ca2+摄取。EM和GM在>0.34mmol·L-1时显著抑制内质网Ca2+摄取(P<0.05或0.01)。结论低浓度EM和GM未能引起胞浆Ca2+升高可能与其促进线粒体Ca2+摄取与抑制内质网Ca2+摄取相互平衡有关;高浓度EM和GM引起胞浆Ca2+升高可能与其均抑制线粒体和内质网Ca2+摄取有关。
Objective To observe the primary mechanism of the effects of gentamicin (EM) and gentamicin (GM) on cytoplasmic Ca2 +. Methods Fura2 / AM probe was used to determine the effect of EM and GM on the plasma Ca2 + of renal epithelial cells in LLCPK1 cells at different concentrations. The effect of EM and GM on the Ca2 + uptake and endoplasmic reticulum Ca2 + uptake were determined by isotope labeling method. Results EM and GM had no significant effect on cytoplasmic Ca2 + concentration at 1 mmol·L-1, P> 0.05. At 10 mmol·L-1, cytosolic Ca2 + increased significantly (P <0.01). EM and GM significantly increased mitochondrial Ca2 + uptake at 1 mmol·L-1 (P <0.05), while at 10 mmol·L-1, both significantly inhibited mitochondrial Ca2 + uptake. EM and GM significantly inhibited the uptake of endoplasmic reticulum Ca2 + (P <0.05 or 0.01) at> 0.34 mmol·L-1. Conclusions The failure of EM and GM to induce cytoplasmic Ca2 + upregulation may be related to its promotion of uptake of mitochondrial Ca2 + and inhibition of endoplasmic reticulum Ca2 + uptake. High concentrations of EM and GM may induce cytoplasmic Ca2 + upregulation, both of which may inhibit mitochondrial and endoplasmic reticulum Mesh Ca2 + uptake.