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取待分离的“五四○六”抗生菌菌种管一支,加入5毫升左右的无菌水,用接种环把孢子刮下并研成孢子悬液,取一环悬液放在含有约0.5毫升的灭菌水的培养皿中,混匀后取出一环移人另一盛无菌水的培养皿中,依此法多稀释几个培养皿,然后在各皿中注入融化并冷却到50℃左右的马铃薯琼脂培养基约15毫升,摇匀、凝固后,翻转培养皿,使皿盖在下置于温箱中培养,待菌落长出后,根据菌种特征,观查有否杂菌污染。从单个菌落分布疏散,菌落之间不相连的培养皿上,选取菌落为圆形较大,呈粉白色略带橙红,色鲜且有多量露珠分泌的,活力较强的“五四○六”抗生菌菌落做为纯种,接入斜面培养基上。如果发现是噬菌体污染,在一般条件下,应把菌种斜面彻底灭菌消毁,房屋、用具彻底消毒灭菌。有条件的也可以进行筛选抗噬菌体的“五四○六”抗生菌菌株,其方法与前面介绍的相同。
To be separated from the “May 4” antibacterial bacteria a tube, add about 5 ml of sterile water, the ring with the inoculation of spores scraped and ground into a spore suspension, take a suspension on the ring containing about 0.5 ml of sterilized water Petri dishes, mix and remove the ring moved to another dish filled with sterile water, according to this method to dilute a few Petri dishes, and then in each dish into the melt and cooled to About 50 ml of potato agar medium about 15 ml, shake, coagulation, the petri dish, flip the dish cover placed in the incubator until the colony grow, according to the characteristics of the species, check whether there are bacteria Pollution. From a single colonies scattered distribution, colonies are not connected between the Petri dishes, select the colony is larger round, pinkish white slightly orange red, fresh color and a large amount of dewdrop secretion, strong vitality “546” Antibiotic colonies as purebred, access to slant culture medium. If it is found phage pollution, under normal conditions, the bacteria should be thoroughly sterilized slope bevelling, houses, utensils thoroughly disinfection and sterilization. Conditions can also be selected for anti-phage “5460” antibacterial strains, the method described above and the same.