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Schultz组化方法显示胆固醇对多种组织都是常用的。但按原方法要费时半月,且因要以冰冻切片作捞片,在染色过程中极易使切片破损,对本即松散的组织捞片,甚至可使得染色反应无法进行。我们对此方法做了重要改进,大大缩短了时程(后半月余减至4～5天),并把捞片改为恒冷切片后直接粘贴于载片或盖片上,大大改善了切片在染色过程中的完整性,使松散组织亦可进行染色。现将改良方法简介如下:1.取新鲜材料恒冷切片20μm,直接敷贴于载片上,空气干燥。2.CCF液
Schultz’s histochemical method shows that cholesterol is commonly used in many tissues. However, according to the original method to take a half-moon, and because of the frozen slice for the fishing film, the slice easily damaged in the dyeing process, which is loose tissue fishing piece, and even can make the dyeing reaction can not be carried out. We have made important improvements to this method, greatly reducing the schedule (after the remaining half months to 4 to 5 days), and changed to salmon slices after the cold slice directly affixed to the slide or cover, greatly improving the slice in The integrity of the dyeing process allows loose tissue to be dyed. Now the improved method is as follows: 1. Take fresh material constant cold section 20μm, apply directly on the slide, air-dried. 2.CCF liquid