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目的:观察脑出血对大鼠第三脑室伸展细胞的影响及人参皂苷的作用。方法:以Wistar大鼠脑基底的尾状核注入Ⅶ型胶原酶制作脑出血模型,应用免疫组化方法,评价指标为胶质原纤维酸性蛋白(GFAP)、一氧化氮合酶(NOS)、巢蛋白(nestin)、碱性成纤维细胞生长因子(b FGF)。结果:脑出血后大鼠第三脑室侧壁及底部的GFAP、NOS、nestin和b FGF阳性细胞数明显增多,可见伸展细胞的长突起伸入脑实质,并到达出血灶。给予人参皂苷后上述指标表达增强,阳性细胞数增多,并与模型组比较具有显著性差异(P<0.05,P<0.01)。结论:第三脑室伸展细胞是胚胎发育后的遗留细胞。脑出血能激活第三脑室伸展细胞,并表现神经干细胞(NSCs)的生物学特性,人参皂苷增强了第三脑室伸展细胞表达,促进其参与脑损伤修复。
Objective: To observe the effect of intracerebral hemorrhage on third stretch cells in rats and the effect of ginsenosides. Methods: The model of intracerebral hemorrhage was made by injecting collagenase type Ⅶ into the caudate nucleus of the basal base of Wistar rats. Immunohistochemical method was used to evaluate the expression of glial fibrillary acidic protein (GFAP), nitric oxide synthase (NOS) Nestin, basic fibroblast growth factor (bFGF). Results: The number of GFAP, NOS, nestin and b FGF positive cells in the lateral wall and bottom of the third ventricle of rats significantly increased after ICH. The long protuberances of extensor cells protruded into parenchyma and reached the hemorrhage foci. After administration of ginsenosides, the expression of these indexes increased, the number of positive cells increased, and compared with the model group had significant difference (P <0.05, P <0.01). CONCLUSION: The third ventricle stretch cells are the remaining cells after embryonic development. Intracerebral hemorrhage can activate the third ventricle stretch cells and express the biological characteristics of neural stem cells (NSCs). Ginsenosides increase the expression of stretch cells in the third ventricle and promote their involvement in brain injury repair.