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目的：探讨环孢素Ａ体外抗曼氏血吸虫的作用。方法：ＭＦ１小鼠实验感染曼氏血吸虫６ｗｋ后，经主动脉和门静脉灌注收虫。将雄虫放入含有１μｇ／ｍｌ、１０μｇ／ｍｌ、１５μｇ／ｍｌ、２０μｇ／ｍｌ和２５μｇ／ｍｌ环孢素Ａ的１９９培养液中体外培养。用扫描电镜对药物所致的虫体皮层损害作时间生物学观察。结果：在体外，雄虫经１μｇ／ｍｌ环孢素Ａ作用后，体嵴的结构疏松；经１０μｇ／ｍｌ环孢素Ａ作用２４ｈ后，雄虫皮层肿胀；雄虫经１５μｇ／ｍｌ环孢素Ａ作用８ｈ－２４ｈ后，虫体皮层破溃；虫体经２０μｇ／ｍｌ药物作用２４ｈ后，雄虫皮层明显破溃；雄虫经２５μｇ／ｍｌ环孢素Ａ作用８ｈ后，皮层褶嵴受损；作用１６ｈ后，皮层肿胀；作用２４ｈ后，皮层极度破溃，皮棘脱落。药物所致的虫体皮层损害与剂量和时间呈依赖关系。结论：环孢素Ａ具有直接杀曼氏血吸虫的作用。
Objective: To investigate the effect of cyclosporin A against Schistosoma mansoni in vitro. Methods: MF1 mice experimentally infected with Schistosoma mansoni 6wk, after the aorta and portal vein perfusion received insects. Male adults were cultured in 199 medium containing 1 μg / ml, 10 μg / ml, 15 μg / ml, 20 μg / ml and 25 μg / ml cyclosporine A in vitro. Scanning electron microscopy of drug-induced parasites cortical damage for biological observation. Results: In vitro, the structure of the cristae was loosened by 1μg / ml cyclosporine A. The cortex became swollen after treated with 10μg / ml cyclosporine A; After 8h-24h of A treatment, the cortex of the parasite ruptured. After the mice were treated with 20μg / ml of drug for 24 hours, the cortex of the male worm was significantly ruptured. After 16 hours, the cortex became swollen; after 24 hours, the cortex became extremely ruptured and the leather spine came off. Drug-induced parasitoids cortical damage dose and time-dependent. Conclusion: Cyclosporin A has a direct role in killing Schistosoma mansoni.