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OBJECTIVE:To investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract(ESC) in vitro.METHODS:ESC was first extracted with ethanol,and then washed using a polyamide column with 60% ethanol.ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions.Subsequently,the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined.RESULTS:The results showed that ESC was rich in isomers of Chamaejasminor,neochamaejasmine and Sikokianin.ESC had significant cytotoxicity against NCI-H157 cells,with an IC 50 of approximately 18.50 μg.mL-1.ESC caused significant increase in total apoptotic rate,the activity of caspase 3 and 8,and Fas protein expression(P<0.05).CONCLUSION:The inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway.
OBJECTIVE: To investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract (ESC) in vitro. METHODS: ESC was first extracted with ethanol, and then washed using a polyamide column with 60% ethanol.ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions. Substituted, the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined .RESULTS: The results showed that ESC was rich in isomers of Chamaejasminor, neochamaejasmine and Sikokianin. ESC had significant cytotoxicity against NCI-H157 cells with an IC 50 of approximately 18.50 μg.mL-1. ESC caused significant increase in total apoptotic rate, the activity of caspase 3 and 8, and Fas protein expression (P <0.05) .CONCLUSION: The inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway.