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目的对香莲外洗液诱导白念珠菌标准耐药菌株恢复对氟康唑敏感前后的差异基因进行转录组学研究。方法采用微量肉汤稀释法测定白念珠菌标准耐药菌株最低抑菌浓度值(minimal inhibitory concentration,MIC),观察香莲外洗液诱导下白念珠菌标准耐药菌株恢复对氟康唑敏感所需的代数;通过转录组测序(RNA-Seq)测定和比较原代耐药株、香莲外洗液诱导下恢复敏感株的转录组情况;应用基因本体(gene ontology,GO)方法分析香莲外洗液诱导下恢复敏感菌株的差异表达基因。结果香莲外洗液诱导下的耐药白念珠菌转种至第6代时恢复对氟康唑的敏感性。转录组测序表明,对比原代耐药株,香莲外洗液诱导下恢复敏感株有165个基因RPKM(reads per kilo bases per million reads)指标表达上调,144个基因RPKM指标表达下调。GO分析发现:上下调基因主要归类于GO:0015903(fluconazole transport)。结论香莲外洗液可诱导耐药白念珠菌恢复对氟康唑的敏感性,推测可能与开启氟康唑转运通路有关。
OBJECTIVE: To carry out transcriptomics studies on the difference genes before and after sensitivity of fluconazole to fluconazole induced by Candida albicans standard strain. Methods The minimal inhibitory concentration (MIC) of standard strains of Candida albicans was determined by micro broth dilution method. The sensitivity of fluconazole to fluconazole was restored by the standard strain of Candida albicans The algebra was analyzed by RNA-Seq. The transcriptome of the sensitive strains was recovered by the primary drug-resistant strains and the lotus lotion. The gene ontology (GO) Differentially expressed genes in sensitive strains recovered by external washings. Results Xianglian external washings resistant Candida albicans to switch to the 6th generation to resume the sensitivity of fluconazole. The transcriptome sequencing showed that compared with the primary drug-resistant strains, the mRNA expression of RPKM was up-regulated in 165 susceptible strains and the expression of 144 genes was down-regulated. GO analysis found that the up-down genes are mainly classified as GO: 0015903 (fluconazole transport). Conclusion The lotus lotion can induce the resistance of Candida-resistant Candida to fluconazole, suggesting that it may be related to the opening of fluconazole transport pathway.