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目的:探讨野生型p53基因抑制膀胱癌生长的作用及其机理.方法:将野生型p53基因重组腺病毒载体转染入膀胱癌细胞HTB9,观察细胞生长曲线、细胞周期变化及DNA合成情况,应用RT—PCR检测p21 mRNA水平,应用免疫组化法检测p21蛋白表达水平.结果:野生型p53基因导入可抑制HTB9细胞生长和DNA合成(AdCMVp53);流式细胞仪显示,DNA合成前期或静止期的细胞比例增高,而合成期细胞比例下降.另外,AdCMVp53还提高了p21的mRNA和蛋白水平.结论:腺病毒载体介导的野生型p53基因转染对于膀胱癌可能是很有前途的基因治疗方法.
Objective: To investigate the role of wild-type p53 gene in bladder cancer growth and its mechanism.Methods: The wild-type p53 gene recombinant adenovirus vector was transfected into bladder cancer cell line HTB9 to observe the cell growth curve, cell cycle and DNA synthesis The expression of p21 protein was detected by RT-PCR and the expression of p21 protein was detected by immunohistochemistry.Results: The wild-type p53 gene could inhibit the growth of HTB9 cells and DNA synthesis (AdCMVp53). Flow cytometry showed that pre-DNA or quiescent While the proportion of cells in the synthesis phase decreased.Additionally, AdCMVp53 also increased the mRNA and protein level of p21.Conclusion: Adenovirus vector mediated wild-type p53 gene transfection may be a promising gene therapy for bladder cancer method.