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目的：探讨人乳头瘤病毒（ＨＰＶ）１６、１８型感染及抑癌基因ｐ５３突变与宫颈癌发生的关系。方法：应用ＰＣＲ及其单链构象多态性（ＰＣＲ－ＳＳＣＰ）方法，对６０例宫颈癌组织ＤＮＡ进行检测，同时选择宫颈糜烂及正常宫颈各５０例作为对照。结果：宫颈癌组织中ＨＰＶ１６感染呈明显梯度关系，且鳞癌组织中ＨＰＶ１６的检出率远比腺癌高（８６．７％，３３．３％，Ｐ＜０．０１），并高于ＣＩＮ和正常宫颈组织；相反，腺癌中ＨＰＶ１８的检出率比鳞癌高（５３．５％，６．７％，Ｐ＜０．０１）。ＰＣＲ－ＳＳＣＰ研究涉及ｐ５３基因外显子５～８的突变，它主要在ＨＰＶ阴性的宫颈癌中出现。结论：宫颈癌发生与人乳头瘤病毒感染及ｐ５３基因功能失活有关。
Objective: To investigate the relationship between human papillomavirus (HPV) type 16 and 18 infection and p53 gene mutation and cervical carcinogenesis. Methods: The DNA of 60 cases of cervical cancer was detected by PCR and single-strand conformation polymorphism (PCR-SSCP), and 50 cases of cervical erosion and normal cervix were selected as control. Results: The HPV16 infection in cervical cancer showed a significant gradient, and the detection rate of HPV16 in squamous cell carcinoma was much higher than that in adenocarcinoma (86.7%, 33.3%, P <0.01) and higher than that in CIN And normal cervical tissue. On the contrary, the detection rate of HPV18 in adenocarcinoma was higher than that in squamous cell carcinoma (53.5%, 6.7%, P <0.01). The PCR-SSCP study involved mutations in exons 5 to 8 of the p53 gene, which predominantly occur in HPV-negative cervical cancers. Conclusion: Cervical cancer is associated with human papillomavirus infection and p53 gene inactivation.