维生素Ｂ12为临床常用抗贫血药,其含量测定方法主要采用紫外分光光度法[1],但由于片剂的主成分含量太少,辅料影响较大,因此,片剂采用紫外分光光度法测定含量准确性与专属性均较差,本文建立了高效液相色谱法测定维生素Ｂ12片的含量,结果准确,专属性好。1　仪器与试剂仪器:岛津Ｌ Vitamin B12 is commonly used in clinical anti-anemia drugs, the determination of the content of the main methods of UV spectrophotometry [1], but because of the main component of the tablet is too small, excipients have a greater impact, therefore, tablets using UV spectrophotometry determination of content Accuracy and specificity are poor, the establishment of a high-performance liquid chromatography determination of vitamin B12 tablets, the results are accurate and good specificity. 1 instruments and reagents equipment: Shimadzu L