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目的研究构棘Cudrania cochinchinensis果实的化学成分。方法采用95%乙醇浸泡提取、硅胶柱色谱分离,各种谱学方法和单晶衍射确定化合物结构,用MTT法进行化合物抗肿瘤活性的筛选。结果从构棘果中分离得到2个苯并吡喃异黄酮,分别鉴定为4′-O-methylalpinmumisoflavone(1)和isoderrone(2);晶体结构显示化合物1和2均属单斜晶系,空间群为P2(1)/c,晶体以分子间氢键、分子内氢键、π-π堆积作用和范德华力构成三维超分子建筑结构。化合物2对肝癌BEL-7404细胞和胃癌SGC-7901细胞具有较好的抑制作用。结论化合物2为首次从该属植物中分离得到,化合物1为首次从该植物中得到。X-单晶衍射测试确定了化合物1和2的平面晶体结构,并首次报道其3D立体堆积结构。
Objective To study the chemical composition of Cudrania cochinchinensis fruit. Methods The compounds were isolated by soaking in 95% ethanol, separated by silica gel column chromatography, all kinds of spectroscopic methods and single crystal diffraction. The antitumor activity was screened by MTT method. Results Two benzopyran isoflavones were isolated from the eucalyptus fruit and identified as 4’-O-methylalpinmumisoflavone (1) and isoderrone (2) respectively. The crystal structure showed that compounds 1 and 2 belong to monoclinic space The group P2 (1) / c, the crystal structure of three-dimensional supramolecular structure by molecular hydrogen bonds, intramolecular hydrogen bonds, π-π stacking and Van der Waals forces. Compound 2 has a good inhibitory effect on BEL-7404 cells and SGC-7901 cells. Conclusion Compound 2 was isolated from this genus for the first time. Compound 1 was obtained from this plant for the first time. X-ray single crystal diffraction tests confirmed the planar crystal structures of compounds 1 and 2, and reported their 3D stereoscopic packing structure for the first time.